 | The new Thermo Scientific Krios G4 Cryo Transmission Electron Microscope (Cryo-TEM) enables you to unravel life at the molecular level—easier, faster, and more reliably than ever before. The most compact TEM in its class, the Krios G4 Cryo-TEM consists of a highly stable 300 kV TEM platform and the industry-leading Autoloader (cryogenic sample manipulation robot), making it ideally suited for automated applications such as single particle analysis (SPA), cryo-electron tomography (cryoET) and micro electron diffraction (MicroED). Through a thorough redesign of the mechanical base frame and system enclosure, the microscope height has been reduced to below 3 meters, which allows for instrument installation in labs with a ceiling height below 3.04m (~10 ft), thereby avoiding costly room renovations. Designed-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing. Cryo-EM productivityAutomated alignment routines ensure the microscope is tuned to its optimal starting point for data acquisition, which itself is accelerated up to 4x through the use of aberration-free image shifting along with the ability to collect more images per hole via a fringe free mode. Additionally, acquired data can be evaluated through on-the-fly monitoring of data quality and optimizing acquisition parameters as needed. | |
The Thermo Scientific Krios Rx Cryo-TEM is the flagship of the iSPA Workflow, the first pharma-dedicated solution for single particle analysis (SPA) and structure-based drug design. This high-end microscope is optimized for the drug discovery process with a throughput of over 400 high-quality images per hour. The Krios Rx Cryo-TEM is configured for SPA only with a fixed voltage (300 kV), and comes standard equipped with a Falcon 4 detector. The simplified workflow with next-level EPU automations, including unattended multigrid data collection, makes it ideal for non-expert microscopists. The pharma-dedicated service package ensures the highest productivity and reliability with a 90% system uptime guarantee.
With a height less than 3 meters and an active vibration dampening system, the microscope fits in a standard room allowing easy installation and avoiding costly room renovations. The automated and programmable loading door also enables compatibility with robotic sample loading in the future.
Designed for stability and speed, the Thermo Scientific Selectris and Selectris X Imaging Filters are post-column imaging filters that improve the contrast of TEM images, resulting in high-resolution structures up to atomic resolution. Paired with the latest generation Thermo Scientific Falcon 4 Direct Electron Detector, Selectris Filters enable you to obtain high-resolution structures quickly, revealing new biological insights. To further increase the productivity and ease-of-use of your cryo-electron microscopy (cryo-EM) research, these filters are fully integrated into Thermo Scientific EPU Software, Thermo Scientific Tomography Software, and instrument operation software. The Selectris and Selectris X Imaging Filters are available on Thermo Scientific Krios and Glacios Cryo-TEMs.
Key features
Improved ergonomicsEasier sample exchange and data acquisition thanks to enhanced automation, systematic user guidance, and advanced performance monitoring. Fits more easily into new and existing labsAvoid costly and challenging renovations with a completely redesigned mechanical base frame and system enclosure, resulting in a system height of <3 m. | Maximized productivityA powerful combination of fringe free imaging (FFI), aberration-free image shift (AFIS), and the (optional) Falcon 4 Detector enhance SPA throughput significantly. AutomationBuilt-in self-diagnostic function (APM) evaluates microscope alignments for acquiring high-resolution data. Integrated Thermo Scientific EPU Software performs automatic daily tuning of essential alignments. | Workflow connectivityFits seamlessly into SPA cryoET, and MicroED workflows, with convenient and contamination-risk-free sample transfer throughout the workflow. Best image quality for high-resolution 3D reconstructionImproved information limit (0.12 nm) and linear distortion below 0.5% ensure the best possible boundary conditions for high-resolution imaging. This can be further improved with the Thermo Scientific Falcon 4 Detector and the Thermo Scientific Phase Plate Solution. |
For more details: https://www.thermofisher.com/tr/en/home/electron-microscopy/products/transmission-electron-microscopes/krios-g4-cryo-tem.html
